| 1. | Research on effects of superfine and common powder of gastrodia elata bl . tianma on neuronal apoptosis in rat with cerebral ir injuries
天麻超微粉与普通粉对大鼠脑缺血再灌注损伤神经细胞凋亡的研究 |
| 2. | From the aflp data , the coefficient between m and gastrodia elata bl . samples was 0 . 546 which was the smallest among coefficients , t3 and t4 which came from the same area have the most familiar relationship with the largest coefficient
5 ； 52条特异性条带可区分出所有要分析的6个牛膝样品；引物opw 17的特异性条带率最高，达85 |
| 3. | Gastrodia elata bl and cyathula officinalis kuan , are two kinds of chinese traditional medicine herbs which are used widely . in the study their dna polymorphisms in them were analyzed by using rapds and aflps
天麻是传统名贵中药材，具有镇静、抗惊厥、抗炎镇痛、抗衰老、提高免疫功能等作用，另外还含有具有广泛应用前景的抗真菌蛋白。 |
| 4. | 300 clear bands including 289 polymorphic bands and 50 specific bands were amplified from the genomic dna of gastrodia elata bl . by these selected primers . the rate of polymorphic bands and the rate of specific bands were 98 . 0 % and 16 . 6 % respectively
6个引物组合共扩增出300条清晰可辨的条带，其中289条多态性条带， 50条特异性条带；多态性条带率和特异性条带率分别达98 |
| 5. | 92 distinct bands including 87 polymorphic bands and 24 specific bands were amplified from the genome dna of gastrodia elata bl . by using these selected primers belong to groups of opb , opi , opn , op w . the rate of polymorphic bands and the rate of specific bands were 93 . 5 % and 25 . 0 % respectively . seven often samples excluding tl , t3 and t9 were identified by the 24 specific bands
随机引物opb - 5扩增出的特异性条带最多，有4条，特异性条带率达57 . 2 ，可以鉴定出m和t8两个样品；随机引物opn - 10扩增出的9条带中，有3条特异性的带，可区分m ， t8 ， t2三个样品。 |
| 6. | Armillaria mellea ( m ) owned the most bands ; t3 had 3 specific bands secondly . m and t8 were distinguished using the 4 specific bands which were amplified through primer opb - 05i m , t8 and t2 were identified by 3 specific bands which were amplified through primer opn - 10 , these amplified specific bands by this primer distinguished the most samples . according to clustering tree plot of gastrodia elata bl
Ntsys软件聚类结果表明：采自同一地方(广元青川)不同地点的t5 ， t6具有最近的亲缘关系，其遗传相似系数达0 . 81 ；密环菌m与其它9种野生与栽培系的天麻的遗传距离最远，其遗传相似系数只有0 . 37 。 |
| 7. | Except the cortex 6 pair of aflp primers which might produce dna polymorphisms from the genomic dna of gastrodia elata bl . were filtered from 145 pair of e / m primers , 92 pair of e / p primers and 72 pair of e / p primers . all selected premiers were pairs of e / p , this did not coincided on the results in other plants , in which pair of e / m or p / m were used as aflp primers
3通过对145对e m引物组合， 92对m p引物组合， 72对e p引物组合进行筛选，仅在e p引物组合中寻找出6组对天麻具多态性扩增且重复性好的引物，与其它植物中用e m或e p组合进行扩增不一致，说明采用aflp技术对天麻等中药材进行研究，其引物组合与其它植物类材料研究相比有较大的差异。 |
| 8. | Samples was only 0 . 37 which was the smallest among coefficients ; tl , t2 , t3 and t4 which came from gaozhuang plantation , mianyang , sichuan , and t5 , t6 , t7 , which came from laohegou planation , mianyang , sichuan , were clustered two groups . the greatly genetic variations in samples of gastrodia elata bl . in different areas and altitude were revealed though the analysis of the tree plot
从总体上看，采自高庄林场的n ， t2 ， t3 ， t4和老河沟采育场的t5 ， t6 ， t7及采自雅安地区的t9 ， t8可以聚为两大类，说明由于地理分布和海拔高度的不同，天麻种内的dna存在较大的变异性。 |
| 9. | Great diversity in genomic dna of gastrodia elata bl . and armillaria mellea . was also demonstrated by completely different bands of the rapd between various growing periods of t8 and m . the complete different bands of the rapd between m and the tuber of t8 might revealed that the genetic matter of m did not invade the inter area of gastrodia elata bl
2通过对同一株不同生长时期的天麻与蜜环菌的rapd扩增结果的研究表明：同一株天麻不同生长时期的dna不表现差异，而且与密环菌没有共同的条带，这说明密环菌与天麻的dna有较大的差异，特别是与块茎之间的差异说明密环菌的遗传物质在天麻的生长过程中侵入天麻块茎皮层以内的部位时已被同化。 |
